pSPgRNA is a plasmid designed for the expression of a guide RNA (gRNA) for CRISPR-Cas9 systems.
Utilizing Addgene Plasmid #47108 (pSPgRNA) for Gene Editing.
Define the role of gRNA in CRISPR/Cas9 systems.
To help me provide more relevant content, could you tell me:
It allows researchers to insert a specific 20-nucleotide guide sequence to target Cas9 to a specific genomic location. Vector Type: Mammalian Expression Vector. Key Components: U6 Promoter: Drives the high-level expression of the gRNA.
Note: The search results also indicate this is part of a larger, often complex data set.
Are you using this for (like at Addgene)?
Expression of the chimeric guide RNA in mammalian cells.
