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While optical microscopes use light and electron microscopes use beams of electrons, Atomic Force Microscopy (AFM) works by "feeling" a surface—much like a record player needle or a person reading Braille. It is one of the most powerful tools in nanotechnology because it doesn't just see atoms; it interacts with them. 1. The Fundamentals: How It Works
The tip hovers just above the surface, sensing long-range forces. This is used for extremely hydrophobic surfaces or when the sample must remain completely untouched. 3. Key Applications
The tip can be used as a "pen" to move individual molecules or etch patterns into a surface, enabling bottom-up nanofabrication . 4. Why Use AFM Over SEM? Unlike Scanning Electron Microscopy (SEM), AFM: Fundamentals and Application of Atomic Force Mi...
The tip "drags" across the surface. It provides high resolution but can damage soft samples like biological tissues.
The core of an AFM is a with a sharp probe (tip) at its end. This tip typically has a radius of curvature on the scale of a few nanometers. While optical microscopes use light and electron microscopes
AFM has moved far beyond simple "pictures." It is now a lab-on-a-tip:
As the tip scanned across a surface, atomic forces (like Van der Waals, electrostatic, or capillary forces) cause the cantilever to deflect. The Fundamentals: How It Works The tip hovers
Researchers use AFM to visualize the double helix of DNA or the movement of protein channels in living cell membranes under physiological conditions (liquid environments).